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time annotating c botulinum atcc 3502 microarray  (ATCC)


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    Structured Review

    ATCC time annotating c botulinum atcc 3502 microarray
    Time Annotating C Botulinum Atcc 3502 Microarray, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+botulinum+atcc+3502/pfizer+Pneumococcal+polysaccharide+powder+Type+2/pmc12754789-216-4-8
    Average 93 stars, based on 4 article reviews
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    93/100 stars

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    other:

    Article Title: Methods for the manufacture of proteolytically processed polypeptides
    Article Snippet: Finally, the precipitate was dissolved in 1.2 ml of PBS, transferred into a microdialysis device (QuixSep, Roth, Germany) and dialysed against PBS at 4° C. The protein content (mg/ml) was analysed by 12.5% SDS-PAGE (FIG. 9A) and measured photometrically at 280 nm and was calculated according to the Lambert-Beer law with an extinction coefficient of 1.33 for IgY. (2) ELISA: Nunc Maxisorp F96 microtiter plates (VWR International GmbH, Darmstadt, Germany) were coated with nBH of various lots (500 ng/mL) in PBS overnight at 4° C. and then blocked for 1 h with blocking buffer of PBS containing 0.1% Tween-20 and 2% nonfat skimmed milk (Merck, Darmstadt, Germany).

    Sequencing:

    Article Title: Methods for the manufacture of proteolytically processed polypeptides
    Article Snippet: .. The genome sequencing of C. botulinum ATCC 3502 has revealed the existence of six ORFs encoding iBH isoforms (Sebaihia et al., 2007, Genome Res. ..

    Control:

    Article Title: Genomic and Phenotypic Characterization of Clostridium botulinum Isolates from an Infant Botulism Case Suggests Adaptation Signatures to the Gut
    Article Snippet: .. C. botulinum ATCC 3502 has two Agr quorum sensing systems, of which the agr-1 operon appears to have a role in sporulation control and the agr-2 operon in positive regulation of neurotoxin production ( ). .. Similar to ATCC 3502, ST7B and related isolates encode conserved agr-1 and agr-2 operons.



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    Gene content differences between <t> Clostridium botulinum </t> isolates CJ4-1 and CJ5-1 identified by DNA microarray analysis.
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    Gene content differences between <t> Clostridium botulinum </t> isolates CJ4-1 and CJ5-1 identified by DNA microarray analysis.
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    Gene content differences between <t> Clostridium botulinum </t> isolates CJ4-1 and CJ5-1 identified by DNA microarray analysis.
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    Gene content differences between  Clostridium botulinum  isolates CJ4-1 and CJ5-1 identified by DNA microarray analysis.

    Journal: Food microbiology

    Article Title: Identification and genetic characterization of Clostridium botulinum serotype A strains from commercially pasteurized carrot juice

    doi: 10.1016/j.fm.2014.05.009

    Figure Lengend Snippet: Gene content differences between Clostridium botulinum isolates CJ4-1 and CJ5-1 identified by DNA microarray analysis.

    Article Snippet: Based on the data presented in this study it appears that CJ5-1 potentially contains four distinct phage regions previously identified in C. botulinum strains ATCC 3502, ATCC 19397 and OkraB.

    Techniques: Microarray, Plasmid Preparation

    (A) PFGE of XhoI (lanes 1–2) and nondigested (lanes 3–4) DNA of Clostridium botulinum carrot juice isolates CJ4-1 (lanes 1 and 3) and CJ5-1 (lanes 2 and 4) and Southern hybridization analysis using (B) Okra17 (C) Okra14 and (D) PL7 gene specific probes.

    Journal: Food microbiology

    Article Title: Identification and genetic characterization of Clostridium botulinum serotype A strains from commercially pasteurized carrot juice

    doi: 10.1016/j.fm.2014.05.009

    Figure Lengend Snippet: (A) PFGE of XhoI (lanes 1–2) and nondigested (lanes 3–4) DNA of Clostridium botulinum carrot juice isolates CJ4-1 (lanes 1 and 3) and CJ5-1 (lanes 2 and 4) and Southern hybridization analysis using (B) Okra17 (C) Okra14 and (D) PL7 gene specific probes.

    Article Snippet: Based on the data presented in this study it appears that CJ5-1 potentially contains four distinct phage regions previously identified in C. botulinum strains ATCC 3502, ATCC 19397 and OkraB.

    Techniques: Hybridization

    (A) PFGE of XhoI (lanes 1–2) and nondigested (lanes 3–4) DNA of Clostridium botulinum carrot juice isolates CJ4-1 (lanes 1 and 3) and CJ5-1 (lanes 2 and 4) and Southern hybridization analysis using (B) BF9 (C) CLB_2952 and (D) B28 gene specific probes.

    Journal: Food microbiology

    Article Title: Identification and genetic characterization of Clostridium botulinum serotype A strains from commercially pasteurized carrot juice

    doi: 10.1016/j.fm.2014.05.009

    Figure Lengend Snippet: (A) PFGE of XhoI (lanes 1–2) and nondigested (lanes 3–4) DNA of Clostridium botulinum carrot juice isolates CJ4-1 (lanes 1 and 3) and CJ5-1 (lanes 2 and 4) and Southern hybridization analysis using (B) BF9 (C) CLB_2952 and (D) B28 gene specific probes.

    Article Snippet: Based on the data presented in this study it appears that CJ5-1 potentially contains four distinct phage regions previously identified in C. botulinum strains ATCC 3502, ATCC 19397 and OkraB.

    Techniques: Hybridization